The start of the agricultural cycle defines the success of the entire production. The quality of germination determines crop uniformity, nutrient absorption, and resistance to stress factors.
In this context, new generation biostimulants, such as AMEN JUICE™, are positioned as high-impact biotechnological tools to improve plant physiology from the seed.
The results obtained in recent experimental trials confirm that AMEN JUICE™ significantly increases the germination percentage in tomato (Solanum lycopersicum), improving initial vigor, stress resistance and the metabolic efficiency of the crop.
This article presents the technical evidence behind this effect and explains the biological mechanisms involved in the process.
Scientific background
AMEN JUICE™ was developed through the integration of cationic biopolymers, natural saponins, and bioactive plant extracts. Its advanced formula is part of the OCST™ (Organic Coating Seed Technology) protocol, designed to restore the seed's biological intelligence and enhance its response to adverse environmental conditions.
Unlike traditional treatments based on synthetic compounds, AMEN JUICE™ works with the molecular structure of the plant and microbial cell membrane, modulating electrical charges and activating endogenous metabolic pathways.
Among its most relevant mechanisms are:
- Electrostatic interaction between cationic biopolymers and negatively charged microbial cell membranes, which promotes selective and active protection.
- Inhibition of fungal growth by blocking RNA synthesis, preventing the proliferation of phytopathogenic fungi in the rhizosphere.
- Activation of the Systemic Acquired Resistance System (SAR), increasing the expression of antioxidant enzymes and defense proteins.
- Improved ion exchange between the seed and the microenvironment, optimizing water absorption and cellular respiration during germination.
These scientific principles support the experimentally observed response: faster, more homogeneous and vigorous germination in seeds treated with AMEN JUICE™.
Study objective: To evaluate the effect of the biostimulant AMEN JUICE™ on the germination percentage, emergence rate and vigor of tomato seedlings under controlled conditions.
Plant material
Commercial seeds of Solanum lycopersicum var. Rio Grande, with high genetic uniformity and without prior treatments, were used.
Treatments
T0 – Control: untreated seeds.
T1 – AMEN JUICE™ (1% v/v): immersion system plus shade drying.
T2 – AMEN JUICE™ (2% v/v): immersion system plus shade drying.
Germination conditions
- Temperature: 25 °C ± 2
- Relative humidity: 75%
- Substrate: filter paper moistened with distilled water
- Light: photoperiod 12 h
- Trial duration: 10 days
Variables evaluated
- Germination percentage (G%)
- Germination rate (VG)
- Radicle length (LR)
- Vigor Index (VIG)
- Percentage of abnormal seedlings
- Visual analysis of fungal mycelium
Results obtained
The results showed a statistically significant improvement in the percentage of germination and vigor in the seeds treated with AMEN JUICE™, especially at the dose of 2% v/v.
| Variable | Control (T0) | AMEN JUICE™ 1% (T1) | AMEN JUICE™ 2% (T2) |
|---|---|---|---|
| Germination (%) | 78 ± 2 | 89 ± 3 | 95 ± 2 |
| Germination rate | 1.0 | 1.3 | 1.6 |
| Radicle length (cm) | 3.1 | 4.5 | 5.2 |
| Vigor Index (VIG) | 242 | 390 | 494 |
The data reflect a 17% increase in total germination and a 50% increase in physiological vigor compared to the control.
The treated seeds also showed a lower presence of fungal colonies, indicating an active antifungal action during germination.
Discussion of results
The observed increase can be attributed to a molecular synergy between the bioactive components of AMEN JUICE™ and the cellular structures of the seed.
Bioelectrostatic interaction
The cationic biopolymer in AMEN JUICE™ interacts with the negative charges of microbial and plant membranes, generating a selective barrier that prevents the penetration of pathogens, but facilitates the diffusion of water and oxygen.
This electrochemical balance is key to maintaining seed viability and accelerating its initial metabolism.
Inhibition of fungal growth
Microscopic experiments showed that fungal structures (especially Fusarium and Rhizoctonia ) reduce their activity when seeds are treated with AMEN JUICE™.
This is because certain components of the biopolymer interfere with RNA synthesis in fungi, limiting their reproduction and colonization.
Metabolic activation
The treated seeds showed an increase in the activity of catalase and peroxidase, antioxidant enzymes associated with initial metabolism and resistance to oxidative stress.
This phenomenon explains the greater radicle length and rapid emergence observed in treatments with AMEN JUICE™.
Optimizing water use
The treatment improves water absorption capacity, acting as a temporary molecular hydrogel that maintains an active moisture film on the seed surface.
This is especially advantageous under conditions of water stress or germination in semi-arid environments.
Agronomic impact
The benefits observed in the experimental phase translate into direct advantages at the production level:
Uniformity in the emergency
Homogeneous germination facilitates agronomic planning, mechanized sowing, and transplanting efficiency.
Less seed loss
Antifungal protection significantly reduces mortality in the early stages, decreasing reseeding costs.
Savings in chemical inputs
The product's preventative action minimizes the need for synthetic fungicides, aligning with regenerative agriculture practices and environmental sustainability.
Greater water and nutritional efficiency
More vigorous root systems optimize the uptake of water and nutrients, contributing to balanced growth and resistance to abiotic stress.
Increase in the quality of the harvest
Initial vigor and early metabolic activation directly impact the size, color, and uniformity of the final fruit.
Technical application considerations
To maximize the observed benefits, the following management practices are recommended:
- Suggested dosage: 1–2% v/v in immersion for 3–5 minutes.
- Drying: in the shade, on an absorbent surface, avoiding temperatures above 35 °C.
- Compatibility: can be combined with inoculants and biological seed treatments.
- Optimal activation pH: ≤ 5.0, the range in which the biopolymer maintains its stable cationic charge.
- Storage: Keep treated seeds in a cool, ventilated environment, no more than 48 hours before sowing.
These conditions guarantee the integrity of the coating and the stability of the bioactive effect.
Biotechnological support of the OCST™
The OCST™ (Organic Coating Seed Technology) protocol of AMEN JUICE™ represents an innovation within agricultural bioengineering.
Its formulation combines:
- Cationic biopolymers: natural derivatives with the ability to interact electrostatically and form a protective film.
- Plant saponins: biological surfactants that increase membrane permeability and enhance germination.
- Antioxidant botanical extracts: which stabilize the membrane and promote cellular respiration.
The combination of these components allows the creation of a self-regulating microenvironment around the seed, acting as an "intelligent system" of biological activation.
In terms of technological innovation, the OCST™ is inspired by principles of nanobiotechnology applied to regenerative agriculture, similar to technologies certified by the FDA and originally developed for aerospace applications by NASA.
Conclusion
Tomato germination trials treated with AMEN JUICE™ demonstrate an average increase of 17% in effective germination and 50% in physiological vigor, confirming its effectiveness as a new generation biostimulant.
This performance is explained by its synergistic action at the molecular level: selective blocking of pathogens, metabolic activation and restoration of the bioelectric balance of the seed.
Beyond the numerical results, the impact of AMEN JUICE™ transcends the agronomic: it promotes a regenerative production model, aligned with NANOAGRO's vision of integrating science, sustainability and ecological awareness in every phase of the production cycle.
Technical references
ClaroShield LLC, Internal Technical Report: “Proactive Control Protocol (PCOP) for Plant Surface Protection” , 2022.
NanoAgro Internal Data Sheet: OCST™ Biopolymer Coating Trials in Tomato Seeds , Panama, 2024.
FAO (2021). Seed Treatment and Germination Enhancement in Sustainable Agriculture .
Hadwiger, L.A. (2015). Plant defense activators and RNA interference in fungal suppression . Plant Science Journal.
NASA Spinoff Database (2020). Nanostructured Surface Coatings in Agricultural Systems .
